Anti Iba1, Rabbit (for Immunocytochemistry)
Product BrandFUJIFILM Wako
Shipping ConditionDry Ice
Iba1 (Ionized calcium-binding adapter molecule1) is an approximately 17 kDa calcium-binding protein. It is used as a microglial marker because it is expressed specifically in microglia in the central nervous system1). It is expressed in both resting and activated microglia, but is reportedly expressed more highly in activated microglia2). It is also expressed in macrophages in peripheral tissues and is known as AIF-1 (Allograft inflammatory factor-1). Iba1 binds to F-actin in cells to form actin bundles. The formation of actin bundles is thought to be required for the membrane ruffling observed during cell migration and phagocytosis3).
FUJIFILM Wako’s "Anti Iba1, Rabbit (for immunocytochemistry)" (Product Number 019-19741), which allows even microglia processes to be stained by immunohistochemical staining, is used by researchers all over the world as a microglia marker antibody standard.
- Enables even the processes of microglia to be immunohistochemical-stained*.
- Relatively small amount needed, with concentrations ranging from 1:500 to 1:1000.
* The result of staining depends on sample condition. Staining performance is not guaranteed.
|Antigen||Synthetic peptide (Iba1 C-terminal sequence)|
|Reactivity||Human, Mouse, Rat, Other*1|
|Concentration (mg/mL)||0.5 - 0.7|
|Applications||Immunohistochemistry (Frozen Section) 1:500 - 1000|
Immunocytochemistry 1:500 - 1000
* Experience with dogs4), cats5), pigs6), marmosets7), zebrafish8), etc. has been reported.