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Serum-Free Cell Freezing Medium

BAMBANKER is a serum-free medium for long-term freezing at -80 degrees C and preservation of valuable culture cells such as tumor cells and normal cells


  • Ready-to-use medium for preservation of cells
  • Usable without diluting
  • No program freezer is required
  • Rapid and long-term freezing and preservation in a deep freezer (-80 C)
  • Serum-free
Operating Procedure
  1. Centrifuge the cells in logarithmic growth phase*, and collect 5 x 10^5 ~ 1 x 10^7 cells.
  2. Suspend the collected cells in 1 mL of this medium, and place the cells in cryotubes for freezing and preservation. Then, freeze and preserve the cells at -80 C without preliminary freezing. The cells frozen at -80 C can be preserved subsequently in liquid nitrogen.
  3. Thawing must be carried out quickly in a constant-temperature chamber or a bath.
Sterility Test**
  • Endotoxin: chromogenic substrate method
  • Mycoplasma: Fluorescent antibody method
  • Fungi and bacteria: As per Japanese Pharmacopoeia
Note* : The cells must be frozen in logarithmic growth phase.**: Certificate of analysis will be issued upon request.

Applications [Cells preserved well by cryopreservation test]:

  • P3U1 (mouse myeloma cell line), K562 (human leukemia cell line), human gastric epithelial cells, etc.
  • Daudi (human B cell line), PC12 (rat-derived adrenal pheochromocytoma), human B cell line
  • OKT4 (mouse hybridoma), monkey B cell line, Activated lymphocyte derived from human peripheral blood, Activated lymphocyte derived from mouse spleen
Manufactured by LYMPHOTEC Inc. (Japan).

Bambanker Publications List

  1. Hikichi T., et al.: Differentiation Potential of Parthenogenetic Embryonic Stem Cells Is Improved by Nuclear Transfer, Stem Cells25: 46-53 (2007).

  2. Thuy BH, et al.: Essential role of paternal chromatin in the regulation of transcriptional activity during mouse preimplantation development. Reproduction141: 67-77 (2011).

  3. Saeki K. et al.: High Efficiency Production of Subculturable Vascular Endothelial Cells from Human Embryonic Stem Cells, Cloning and Stem Cells4: 509-22 (2009).

  4. Takata Y. et al.: Generation of iPS cells using a BacMam multigene expression system. Cell Struct Funct., 36(2): 209-222 (2011)

  5. Zaidi S. et al.: Runx2 deficiency and defective subnuclear targeting by pass senescence to promote immortalization and tumorigenic potential. PNAS., 104(50): 19861-19866 (2007)

  6. Miono S., et al.: Characteristics and Function of Cryopreserved Bone Marrow-Derived Endothelial Progenitor Cells. Annals of Thoracic Surgery., 85: 1361-6 (2008)

  7. Hatoya S., et al.: Effect of co-culturing with embryonic fibroblasts on IVM, IVF and IVC of canine oocytes, Journal of Theriogenology66: 1083-1090(2006)

  8. Shimizu Y. et al.: Impaired Tax-specific T-cell responses with insufficient control of HTLV-1 in a subgroup of individuals at asymptomatic and smoldering stages: Cancer Sci.100 (3): 481-9 (2009)

  9. Sato D., et al.,: Tonsillar TLR9 expression and efficacy of tonsillectomy with steroid pulse therapy in IgA nephropathy patients., Nephrology Dialysis Transplantation, (2011)
  10. T-Cell

  11. Park HS, et al.: Bone marrow T Cells are superior to splenic T cells to induce chimeric conversion after non-myeloablative bone marrow transplantation, Korean J. Intern Med.24 (3): 252-262 (2009)

  12. Huang YH, et al.: Dental Stem Cells and Tooth Banking for Regenerative Medicine, Journal of Experimental & Clinical Medicine2 (3): 111-117 (2010)

  13. Lee SY, et al.: Effects of Cryopreservation of Intact Teeth on the Isolated Dental Pulp Stem Cells, Journal of Endodontics36 (8): 1336-1340 (2010)

  14. Huang MS, et al., Effects of transportation time after extraction on the magnetic cryopreservation of pulp cells of rat dental pulp. Journal of Dental Sciences6(1): 48-52 (2011))

  15. Agata H., et al., Effect of ischemic culture conditions on the survival and differentiation of porcine dental pulp-derived cells, Differentiation76 (9): 981-93 (2008)

  16. Kamada H., et al., In-vitro and in-vivo study of periodontal ligament cryopreserved with a magnetic field, American Journal of Orthodontics & Dentofacial Orthopedics140 (6): 799-805 (2011)

  17. Mieno S., et al., Effects of diabetes mellitus on VEGF-induced proliferation response in bone marrow derived endothelial progenitor cells. J. Card Surg., 25 (5): 618-25 (2010)

  18. Oshima N., et al., Optimized method for culturing outgrowth endothelial progenitor cells, inflammation and Regeneration31 (2): 219-227 (2011)

  19. Liu DG et al, Relation between human decay-accelerating factor (hDAF) expression in pig cells and inhibition of human serum anti-pig cytotoxicity: value of highly expressed hDAF for xenotransplantation, Xenotransplantation, (1): 67-73 (2007)

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