ELISA (Enzyme-Linked Immunosorbent Assay) kits are pivotal tools in biotechnological and medical research, enabling scientists to detect the presence of specific proteins, antibodies, or hormones in a sample. These kits function based on the principle of antigen-antibody interaction. In a typical ELISA, a target molecule (antigen) is affixed to a surface. When a matching antibody, labeled with an enzyme, binds to the antigen, a substrate solution is added. The ensuing enzyme-substrate reaction produces a detectable signal, commonly a color change, revealing the presence and sometimes quantity of the target molecule. Researchers favor ELISA kits for their specificity, sensitivity, and versatility, rendering them instrumental in areas like diagnostics, vaccine development, and pathology. With standardized procedures and high repeatability, ELISA kits streamline research, ensuring consistent and reliable results.